Gallery: HS-AFM Videos
Ice crystallization on mica Temperature variation from +1° to -10°C
Properties:Line Rate (LR): 80 Hz
Image Rate (IR): 0.4 Hz
Scan Size (S): 5 µm x 5 µm
Resolution (R): 200 x 200 pixels
AFM Tip Speed (TS): 0.80 mm/s
AFM Probe: NanoWorld Arrow™ UHF
Video by Keysight Technologies (
www.keysight.com) with Quick Scan on a 9500 Atomic Force Microscope (AFM)
» PLAY VIDEO
Live cell (COS7) - Video 2 Properties: Mode: Phase Modulation Mode
Line Rate (LR): 60 Hz
Image Rate (IR): 0.2 Hz
Scan Size (S): 4 µm x 3 µm
Resolution (R): 320 x 240 pixels
AFM Tip Speed (TS): 0.48 mm/s
AFM Probe: USC-F0.8-k0.1 (customized)
Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Live cell (COS7) - Video 1 Properties: Mode: Phase Modulation Mode
Line Rate (LR): 60 Hz
Image Rate (IR): 0.2 Hz
Scan Size (S): 4 µm x 3 µm
Resolution (R): 320 x 240 pixels
AFM Tip Speed (TS): 0.48 mm/s
AFM Probe: USC-F0.8-k0.1 (customized)
Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Grating with 22 nm Step Height Properties: Mode: Dynamic Mode (Non-Contact)
Line rate (LR): variable (from 50 Hz to 110 Hz)
Image rate (IR): variable (from 0.4 Hz to 0.85 Hz)
Scan Size (S): 5 µm x 5 µm
Resolution (R): 128 x 128 pixels
AFM Tip Speed (TS): variable (from 0.50 mm/s to 1.10 mm/s)
AFM Probe: NanoWorld Arrow™ UHF
Video by Anfatec Instruments AG (
www.anfatec.de) on a Anfatec Level-AFM with AFT-HSC50 controller
» PLAY VIDEO
Streptavidin crystal on a biotin containing lipid bilayerProperties:Mode: Tapping Mode
Line Rate (LR): 600 Hz
Image Rate (IR): 1 Hz
Scan Size (S): 0.03 μm x 0.03 μm
Resolution (R): 120 x 120 pixels
AFM Tip Speed (TS): 0.04 mm/s
AFM Probe: NanoWorld USC-F1.2-k0.15
Video by A. Karner and J. Preiner, Center for Advanced Bioanalysis (CBL), Linz, Austria
» PLAY VIDEO
Exocytic event and Cytoskeleton movement in COS7 live cellProperties:Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
AFM Tip Speed (TS): 0.14 mm/s
AFM Probe: USC-F0.8-k0.1 (customized)
Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Live cell (COS7) Mitochondria-GFPProperties:Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
AFM Tip Speed (TS): 0.14 mm/s
AFM Probe: USC-F0.8-k0.1 (customized)
Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
DNA Origami in bufferProperties:Mode: Amplitude Modulation Mode in buffer
Line Rate (LR): 105 Hz
Image Rate (IR): 0.41 Hz
Scan Size (S): 0.05 μm x 0.05 μm
Resolution (R): 256 x 256 pixels
AFM Tip Speed (TS): 0.01 mm/s
AFM Probe: NanoWorld USC-F0.3-k0.3
Video by JPK Instruments (
www.jpk.com) on a NanoWizard ULTRA Speed AFM
Sample courtesy of Dr. Rebecca Meyer, Prof. Christof M. Niemeyer, Karlsruhe Institute of Technology (KIT), Germany
» PLAY VIDEO
Polyhydroxybutyrate-co-valerate (PHB/V) spherulite crystallization growth frontProperties:Mode: Amplitude Modulation Mode
Line Rate (LR): 60 Hz
Image Rate (IR): 0.24 Hz (played at 5.6 Hz)
Scan Size (S): 1.5 μm x 1.5 μm
Resolution (R): 512 x 256 pixels
AFM Tip Speed (TS): 0.18 mm/s
AFM Probe: SD-USC-Si-2.2MHz
Video by JPK Instruments (
www.jpk.com) on a NanoWizard ULTRA Speed AFM
Sample courtesy of Prof. Jamie Hobbs, University of Sheffield, UK
» PLAY VIDEO
Single-Molecules Imaging of micro-Brownian Movement in a Chiral Helical (pi)-Conjugated PolymerProperties:Mode: Dynamic Mode
Line Rate (LR): 240 Hz
Image Rate (IR): 2.0 Hz (played at 30 Hz)
Scan Size (S): 0.4 μm x 0.3 μm
Resolution (R): 128 x 96 pixels
AFM Tip Speed (TS): 0.19 mm/s
AFM Probe: NanoWorld USC-F1.2-k0.15
Video by M. Grewal and K. Shinohara, JAIST, Japan
» PLAY VIDEO
Calcite crystal growthProperties:Mode: Phase Modulation Mode
Line Rate (LR): 100 Hz
Image Rate (IR): 1 Hz
Scan Size (S): 0.01 μm x 0.005 μm
Resolution (R): 200 x 100 pixels
AFM Tip Speed (TS): 0.002 mm/s
AFM Probe: NanoWorld USC-F5-k30
Video by K. Miyata, H. Asakawa and T. Fukuma, Kanazawa University, Japan. Applied Physics Letters 103 (2013) 203104
» PLAY VIDEO
Lamellipodium on COS7 with USC movementProperties:Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
AFM Tip Speed (TS): 0.14 mm/s
AFM Probe: NanoWorld USC-F1.2-k0.15
Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Lamellipodium on COS7Properties:Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
AFM Tip Speed (TS): 0.14 mm/s
AFM Probe: NanoWorld USC-F1.2-k0.15
Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Lamellipodium on stained COS7Properties:Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
AFM Tip Speed (TS): 0.14 mm/s
AFM Probe: NanoWorld USC-F1.2-k0.15
Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Lamellipodium on non stained COS7Properties:Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
AFM Tip Speed (TS): 0.14 mm/s
AFM Probe: NanoWorld USC-F1.2-k0.15
Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
OmpF trimersProperties:Mode: Non-Contact Mode
Line Rate (LR): 1258 Hz
Image Rate (IR): 2.09 Hz
Scan Size (S): 0.075 μm x 0.075 μm
Resolution (R): 300 x 300 pixels
AFM Tip Speed (TS): 0.19 mm/s
AFM Probe: NanoWorld USC-F1.5-k0.6
Video by I. Casuso and S. Scheuring, U1006 INSERM, Aix-Marseille University, France
» PLAY VIDEO
CELGARDProperties:Mode: Non-Contact Mode
Line Rate (LR): 10 Hz
Scan Size (S): 1 μm x 1 μm
Resolution (R): 400 x 400 pixels
AFM Tip Speed (TS): 0.02 mm/s
AFM Probe: NanoWorld Arrow™ UHF
Video by AIST-NT (
www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
CELGARDProperties:Mode: Non-Contact Mode
Line Rate (LR): 15 Hz
Scan Size (S): 1 μm x 1 μm
Resolution (R): 400 x 400 pixels
AFM Tip Speed (TS): 0.03 mm/s
AFM Probe: NanoWorld Arrow™ UHF
Video by AIST-NT (
www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
HOPG Properties: Mode: Non-Contact Mode
Line Rate (LR): variable (from 10 Hz to 60 Hz)
Scan Size (S): 8 μm x 8 μm
Resolution (R): 512 x 512 pixels
AFM Tip Speed (TS): variable (from 0.16 mm/s to 0.96 mm/s)
AFM Probe: NanoWorld Arrow™ UHF
Video by AIST-NT (
www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
HOPG Properties: Mode: Non-Contact Mode
Line Rate (LR): 30 Hz
Scan Size (S): 8 μm x 8 μm
Resolution (R): 512 x 512 pixels
AFM Tip Speed (TS): 0.48 mm/s
AFM Probe: NanoWorld Arrow™ UHF
Video by AIST-NT (
www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
HOPG Properties: Mode: Non-Contact Mode
Line Rate (LR): 50 Hz
Scan Size (S): 8 μm x 8 μm
Resolution (R): 512 x 512 pixels
AFM Tip Speed (TS): 0.80 mm/s
AFM Probe: NanoWorld Arrow™ UHF
Video by AIST-NT (
www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
HOPG Properties: Mode: Non-Contact Mode
Line Rate (LR): 70 Hz
Scan Size (S): 8 μm x 8 μm
Resolution (R): 512 x 512 pixels
AFM Tip Speed (TS): 1.12 mm/s
AFM Probe: NanoWorld Arrow™ UHF
Video by AIST-NT (
www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
DVD topography Properties: Mode: Contact Mode
Line Rate (LR): 796 Hz
Image Rate (IR): 6.86 Hz
Scan Size (S): variable (from 1.6 μm x 1.6 μm to 6.8 μm x 6.8 μm)
Resolution (R): 100 x 100 pixels
AFM Tip Speed (TS): variable (from 2.55 mm/s to 10.83 mm/s)
AFM Probe: NanoWorld USC-F2-k3
Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, German
» PLAY VIDEO
DVD topography Properties: Mode: Contact Mode
Line Rate (LR): 1243 Hz
Image Rate (IR): 8.4 Hz
Scan Size (S): variable (from 0.78 μm x 0.78 μm to 5.2 μm x 5.2 μm)
Resolution (R): 128 x 128 pixels
AFM Tip Speed (TS): variable (from 1.94 mm/s to 12.93 mm/s)
AFM Probe: NanoWorld USC-F2-k3
Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany
» PLAY VIDEO
DVD topography Properties: Mode: Contact Mode
Line Rate (LR): 1592 Hz
Image Rate (IR): 14 Hz
Scan Size (S): variable (from 1.15 μm x 1.15 μm to 6 μm x 6 μm)
Resolution (R): 100 x 100 pixels
AFM Tip Speed (TS): variable (from 3.66 mm/s to 19.10 mm/s)
AFM Probe: NanoWorld USC-F2-k3
Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany
» PLAY VIDEO
Dissolution of a calcite crystal in diluted HCl Properties: Mode: Contact Mode
Line Rate (LR): 1000 Hz
Image Rate (IR): 10 Hz
Scan Size (S): 1 μm x 1 μm
Resolution (R): 100 x 400 pixels
AFM Tip Speed (TS): 2.00 mm/s
AFM Probe: SD-USC-Si-1.1MHz
Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany
» PLAY VIDEO
Dissolution of a calcite crystal in diluted HCl Properties: Mode: Contact Mode
Line Rate (LR): 1300 Hz
Image Rate (IR): 13 Hz
Scan Size (S): 1 μm x 1 μm
Resolution (R): 100 x 400 pixels
AFM Tip Speed (TS): 2.60 mm/s
AFM Probe: SD-USC-Si-1.1MHz
Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany
» PLAY VIDEO
Actin filaments Properties: Mode: High Speed Scanning AFM
Area: 250×250 nm2
Pixel: 600×120
Frame rate: 0.8 s/frame (1.25 fps)
Play back rate: 5 fps (×4 play)
Buffer: 100 mM KCl, 2 mM MgCl2, 1 mM EGTA, 20 mM Imidazole-HCl, pH7.6
AFM Probe: NanoWorld® USC-F1.2-k0.15
Video by Prof. Noriyuki Kodera, nanoLSI, Kanazawa University, Japan
» PLAY VIDEO